High-performance liquid chromatography (HPLC) is a critical analytical technique for assessing oligonucleotide purity, yet conventional sample-preparation steps can create significant bottlenecks in high-throughput environments. This technical note describes methods to streamline HPLC sample preparation by reducing manual handling and processing time while maintaining analytical accuracy. Key topics addressed include:
- HPLC sample preparation challenges
- Lyophilization integration in workflows
- Reducing analytical bottlenecks
- Improving throughput and consistency
By simplifying workflows and improving throughput, these approaches enable laboratories to accelerate analysis, reduce variability, and better support large-scale oligonucleotide manufacturing and quality control.