Trehalose Sucrose and Glycerol in Freeze-Drying and Storage Stability of Mycobacterium Bovis

The prerequisite of cold chain storage is a challenge for the global vaccination effort. For tuberculosis, live attenuated M. bovis, marketed as BCG vaccine is the only FDA-approved vaccine against this disease. Sodium glutamate remains a preferred and complacent stabilizer in freeze-dried M. bovis with a reported viable cells between 3% to 66% after storage for 28 days at room temperature and 37 °C. Our work here aims to reduce the variability and to further improve the bacterial viability. This webinar gives an overview to an exploratory study which investigated the effect of disaccharides and polyol, in combination to enhance the stability of freeze-dried M. bovis. The composition, recovery (after freeze-drying), survivability (after storage), thermal properties and cake appearance will be discussed. Presented by Dr. Norman Chieng, PhD Formulation Scientist at the Department of Pharmaceutical Technology, International Medical University